FLAGELLA-STAINING ACETIC ACID BACTERIA

Use of malachite green for staining flagella in bacteria.

Preparations are prepared, using completely clean slides and aqueous suspensions of young bacterial agar cultures. A drop of the suspension is placed on one end of the slide; the slide is then tipped, making the suspension drain down to the opposite end (Society of American Bacteriologists, Manual of Microbiological Methods, McGraw-Hill Book Co., New York, 1957). Both the mordanting and the sta...

Deoxyribonucleic Acid Hybrids of Acetic Acid Bacteria.

De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Deoxyribonucleic acid hybrids of acetic acid bacteria. J. Bacteriol. 88:937-945. 1964.-Deuterated N(15)-labeled deoxyribonucleic acid (DNA) from Acetobacter aceti (mesoxydans 4) forms hybrids with ordinary DNA from other species of this genus (A. xylinum, A. pasteurianus, A. estunensis, and possibly A. xylinoides) when the guanine ...

Staining bacterial flagella easily.

A wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. Although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.

Coenzyme linked dehydrogenases of acetic acid bacteria.

Utilization of ethanol by acetic acid bacteria.

It is well known that the acetic acid bacteria can rapidly oxidize ethanol to acetic acid. But whether ethanol can serve as a source of carbon and energy for these bacteria has not been clearly established. Pasteur (1868) noted that Mycoderma aceti which is undoubtedly Acetobacter aceti (Frateur, 1950) can grow well in ethanol media only if acetic acid is also present. Addition of one or two pe...